Tryptophan in the Pore of the Mechanosensitive Channel MscS: ASSESSMENT OF PORE CONFORMATIONS BY FLUORESCENCE SPECTROSCOPY

Structural changes in channel proteins give critical insights required for understanding the gating transitions that underpin function. Tryptophan (Trp) is uniquely sensitive to its environment and can be used as a reporter of conformational changes. Here, we have used site-directed Trp insertion wi...

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Detalhes bibliográficos
Main Authors: Rasmussen, Tim, Edwards, Michelle D., Black, Susan S., Rasmussen, Akiko, Miller, Samantha, Booth, Ian R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2010
Assuntos:
Membrane Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2820766/
https://ncbi.nlm.nih.gov/pubmed/20037156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.071472
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