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Expression of the bisphosphatase domain of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli.

The fructose-2,6-bisphosphatase domain of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (EC 2.7.105/EC 3.1.3.46) was expressed in Escherichia coli by using an expression system based on bacteriophage T7 RNA polymerase. The protein was efficiently expressed (i) as a fusion protein th...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Tauler, A, Rosenberg, A H, Colosia, A, Studier, F W, Pilkis, S J
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282033/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2842783/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.18.6642
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