Expression of the bisphosphatase domain of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in Escherichia coli.
The fructose-2,6-bisphosphatase domain of rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (EC 2.7.105/EC 3.1.3.46) was expressed in Escherichia coli by using an expression system based on bacteriophage T7 RNA polymerase. The protein was efficiently expressed (i) as a fusion protein th...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
|---|---|
| Principais autores: | , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1988
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282033/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2842783/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.18.6642 |
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