Sensitive homologous recombination strand-transfer assay: partial purification of a Drosophila melanogaster enzyme and detection of sequence effects on the strand-transfer activity of RecA protein.

A sensitive homologous recombination strand-transfer assay is described that employs short radiolabeled double-stranded DNA fragments from the lac/polylinker region of plasmid pUC18 and (+)viral M13mp18 single-stranded DNA as substrates. Substitution of a short radiolabeled double-stranded fragment...

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Autors principals: McCarthy, J G, Sander, M, Lowenhaupt, K, Rich, A
Format: Artigo
Idioma:Inglês
Publicat: 1988
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC281863/
https://ncbi.nlm.nih.gov/pubmed/3137561
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