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Directed evolution of copper nitrite reductase to a chromogenic reductant
Directed evolution methods were developed for Cu-containing nitrite reductase (NiR) from Alcaligenes faecalis S-6. The PCR cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. The high-throughput...
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| Autors principals: | , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2010
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2816606/ https://ncbi.nlm.nih.gov/pubmed/20083495 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzp084 |
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