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Facile Identification and Quantitation of Protein Phosphorylation via β-Elimination and Michael Addition with Natural Abundance and Stable Isotope Labeled Thiocholine
Herein, we employ the unique chemical properties of the quaternary amine present in thiocholine (2-mercapto-N,N,N-trimethyl-ethanaminium) in conjunction with alkaline β-elimination and Michael addition (BEMA) reactions for the specific detection, identification and quantitation of phosphorylated ser...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2813211/ https://ncbi.nlm.nih.gov/pubmed/20000356 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac9015193 |
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