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Expression of catalytically active recombinant Helicobacter pylori urease at wild-type levels in Escherichia coli.

The genes encoding Helicobacter pylori urease, a nickel metalloenzyme, have been cloned and expressed in Escherichia coli. Enzymatic activity, however, has been very weak compared with that in clinical isolates of H. pylori. Conditions under which near wild-type urease activity was achieved were dev...

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Autors principals: Hu, L T, Mobley, H L
Format: Artigo
Idioma:Inglês
Publicat: 1993
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC280885/
https://ncbi.nlm.nih.gov/pubmed/8500893
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