An Automated and Multiplexed Method for High Throughput Peptide Immunoaffinity Enrichment and Multiple Reaction Monitoring Mass Spectrometry-based Quantification of Protein Biomarkers

There is an urgent need for quantitative assays in verifying and validating the large numbers of protein biomarker candidates produced in modern “-omics” experiments. Stable isotope standards with capture by anti-peptide antibodies (SISCAPA) has shown tremendous potential to meet this need by combin...

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Autores principales: Whiteaker, Jeffrey R., Zhao, Lei, Anderson, Leigh, Paulovich, Amanda G.
Formato: Artigo
Lenguaje:Inglês
Publicado: The American Society for Biochemistry and Molecular Biology 2010
Materias:
Research
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2808264/
https://ncbi.nlm.nih.gov/pubmed/19843560
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900254-MCP200
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