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Recombination-induced tag exchange to track old and new proteins
The dynamic behavior of proteins is critical for cellular homeostasis. However, analyzing dynamics of proteins and protein complexes in vivo has been difficult. Here we describe recombination-induced tag exchange (RITE), a genetic method that induces a permanent epitope-tag switch in the coding sequ...
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| Autores principales: | , , , , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2009
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2806724/ https://ncbi.nlm.nih.gov/pubmed/20018668 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0911164107 |
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