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Recombination-induced tag exchange to track old and new proteins

The dynamic behavior of proteins is critical for cellular homeostasis. However, analyzing dynamics of proteins and protein complexes in vivo has been difficult. Here we describe recombination-induced tag exchange (RITE), a genetic method that induces a permanent epitope-tag switch in the coding sequ...

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Detalhes bibliográficos
Main Authors: Verzijlbergen, Kitty F., Menendez-Benito, Victoria, van Welsem, Tibor, van Deventer, Sjoerd J., Lindstrom, Derek L., Ovaa, Huib, Neefjes, Jacques, Gottschling, Daniel E., van Leeuwen, Fred
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2009
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2806724/
https://ncbi.nlm.nih.gov/pubmed/20018668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0911164107
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