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Purification, crystallization and preliminary crystallographic studies of the complex of interferon-λ1 with its receptor

Human interferon-λ1 (IFN-λ1(Ins)) and the extracellular domain of interferon-λ1 receptor (IFN-λ1R1) were expressed in Drosophila S2 cells and purified to homogeneity. Both IFN-λ1(Ins) and interferon-λ1 produced from Escherichia coli (IFN-λ1(Bac)) were coupled with IFN-λ1R1 at room temperature and th...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Magracheva, Eugenia, Pletnev, Sergei, Kotenko, Sergei, Li, Wei, Wlodawer, Alexander, Zdanov, Alexander
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2009
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2805539/
https://ncbi.nlm.nih.gov/pubmed/20057073
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109048817
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