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Nonenzymatic sequence-specific methyl transfer to single-stranded DNA.

2'-Deoxyuridine 5'-triphosphate with a methylthioether moiety at the 5 position can be incorporated into a primer-template DNA complex with Klenow enzyme. Activation with CNBr at 25 degrees C, pH 5.5, followed by treatment with piperidine produces sequence-specific cleavage on the template...

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Bibliografische gegevens
Hoofdauteurs: Iverson, B L, Dervan, P B
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1988
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC280485/
https://ncbi.nlm.nih.gov/pubmed/3387429
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