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Nonenzymatic sequence-specific methyl transfer to single-stranded DNA.

2'-Deoxyuridine 5'-triphosphate with a methylthioether moiety at the 5 position can be incorporated into a primer-template DNA complex with Klenow enzyme. Activation with CNBr at 25 degrees C, pH 5.5, followed by treatment with piperidine produces sequence-specific cleavage on the template DNA predo...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Iverson, B L, Dervan, P B
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC280485/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3387429/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.13.4615
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