Nonenzymatic sequence-specific methyl transfer to single-stranded DNA.
2'-Deoxyuridine 5'-triphosphate with a methylthioether moiety at the 5 position can be incorporated into a primer-template DNA complex with Klenow enzyme. Activation with CNBr at 25 degrees C, pH 5.5, followed by treatment with piperidine produces sequence-specific cleavage on the template DNA predo...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
|---|---|
| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1988
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC280485/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3387429/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.13.4615 |
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