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Purification, crystallization and preliminary X-ray analysis of a deletion mutant of a major buckwheat allergen

A 16 kDa buckwheat protein (BWp16) is a major allergen responsible for immediate hypersensitivity reactions including anaphylaxis. A deletion mutant of BWp16 (rBWp16ΔN) was overproduced and purified and was shown to be immunologically active. A three-wavelength MAD data set was collected from a crys...

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Detalhes bibliográficos
Main Authors: Kezuka, Yuichiro, Itagaki, Takashi, Satoh, Rie, Teshima, Reiko, Nonaka, Takamasa
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2802877/
https://ncbi.nlm.nih.gov/pubmed/20054125
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109043127
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