Optimization of Streptomyces bacteriophage φC31 integrase system to prevent post integrative gene silencing in pulmonary type II cells

φC31 integrase has emerged as a potent tool for achieving long-term gene expression in different tissues. The present study aimed at optimizing elements of φC31 integrase system for alveolar type II cells. Luciferase and β-galactosidase activities were measured at different time points post transfec...

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Bibliografiske detaljer
Main Authors: Aneja, Manish Kumar, Geiger, Johannes, Imker, Rabea, Üzgün, Senta, Kormann, Michael, Hasenpusch, Guenther, Maucksch, Christof, Rudolph, Carsten
Format: Artigo
Sprog:Inglês
Udgivet: Korean Society of Medical Biochemistry and Molecular Biology 2009
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Original Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2802687/
https://ncbi.nlm.nih.gov/pubmed/19745601
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3858/emm.2009.41.12.098
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