Accurate and efficient pre-mRNA splicing in Drosophila cell-free extracts.
Synthetic mRNA precursors from the Drosophila fushi tarazu (ftz) gene were shown to be accurately and efficiently spliced in Drosophila nuclear extracts derived from Kc tissue culture cells or 0- to 12-hr embryos. Splicing the ftz pre-mRNA requires ATP and low levels of Mg2+. The reaction proceeds w...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autor principal: | |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1988
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC280111/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3362856/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.9.2904 |
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