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Switching from an induced fit to a lock and key mechanism in an aminoacyl-tRNA synthetase with modified specificity

Methionyl-tRNA synthetase (MetRS) specifically binds its methionine substrate in an induced fit mechanism, with methionine binding causing large rearrangements. Mutated MetRS able to efficiently aminoacylate the methionine (Met) analog azidonorleucine (Anl) have been identified by saturation mutagen...

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Main Authors: Schmitt, Emmanuelle, Tanrikulu, I. Caglar, Yoo, Tae Hyeon, Panvert, Michel, Tirrell, David A., Mechulam, Yves
Formato: Artigo
Idioma:Inglês
Publicado: 2009
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2801061/
https://ncbi.nlm.nih.gov/pubmed/19837083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.10.016
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