Fluorescence-activated cell analysis and sorting of viable mammalian cells based on beta-D-galactosidase activity after transduction of Escherichia coli lacZ.

We demonstrate that individual cells infected with and expressing a recombinant retrovirus carrying the Escherichia coli beta-galactosidase gene (lacZ) can be viably stained, analyzed, sorted, and cloned by fluorescence-activated cell sorting based on the levels of lacZ expressed. To accomplish this...

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Detaylı Bibliyografya
Asıl Yazarlar: Nolan, G P, Fiering, S, Nicolas, J F, Herzenberg, L A
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1988
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC280046/
https://ncbi.nlm.nih.gov/pubmed/3128790
Etiketler: Etiketle
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