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Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue
The β-galactosidase gene (lacZ) of Escherichia coli is widely used as a reporter gene. The expression of lacZ can be detected by enzyme-based histochemical staining using chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-β-d-galactoside (X-gal). Because the enzymatic activity of lacZ is vuln...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer-Verlag
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3353101/ https://ncbi.nlm.nih.gov/pubmed/22371055 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00418-012-0936-1 |
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