Characterization of Endoplasmic Reticulum-Localized UDP-d-Galactose: Hydroxyproline O-Galactosyltransferase Using Synthetic Peptide Substrates in Arabidopsis

We characterized peptidyl hydroxyproline (Hyp) O-galactosyltransferase (HGT), which is the initial enzyme in the arabinogalactan biosynthetic pathway. An in vitro assay of HGT activity was established using chemically synthesized fluorescent peptides as acceptor substrates and extracts from Arabidop...

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Detalhes bibliográficos
Main Authors: Oka, Takuji, Saito, Fumie, Shimma, Yoh-ichi, Yoko-o, Takehiko, Nomura, Yoshiyuki, Matsuoka, Ken, Jigami, Yoshifumi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Plant Biologists 2010
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2799367/
https://ncbi.nlm.nih.gov/pubmed/19923238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.109.146266
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