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Isolation and characterization of the apical surface of polarized Madin-Darby canine kidney epithelial cells.

We have developed a fast and efficient method to isolate the apical surface of Madin-Darby canine kidney epithelial cells. After confluent cell monolayers were coated with alternate layers of cationized colloidal silica and a polyanion, 60% of the apical surface was recovered as large membrane sheet...

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Bibliografische gegevens
Gepubliceerd in:Proc Natl Acad Sci U S A
Hoofdauteurs: Sambuy, Y, Rodriguez-Boulan, E
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 1988
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Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279806/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3278317/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.5.1529
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