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Isolation and characterization of the apical surface of polarized Madin-Darby canine kidney epithelial cells.

We have developed a fast and efficient method to isolate the apical surface of Madin-Darby canine kidney epithelial cells. After confluent cell monolayers were coated with alternate layers of cationized colloidal silica and a polyanion, 60% of the apical surface was recovered as large membrane sheet...

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Detalhes bibliográficos
Main Authors: Sambuy, Y, Rodriguez-Boulan, E
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC279806/
https://ncbi.nlm.nih.gov/pubmed/3278317
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