Isolation and characterization of the apical surface of polarized Madin-Darby canine kidney epithelial cells.
We have developed a fast and efficient method to isolate the apical surface of Madin-Darby canine kidney epithelial cells. After confluent cell monolayers were coated with alternate layers of cationized colloidal silica and a polyanion, 60% of the apical surface was recovered as large membrane sheet...
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1988
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279806/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3278317/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.5.1529 |
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