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High-voltage electroporation of bacteria: genetic transformation of Campylobacter jejuni with plasmid DNA.

Electroporation permits the uptake of DNA by mammalian cells and plant protoplasts because it induces transient permeability of the cell membrane. We investigated the utility of high-voltage electroporation as a method for genetic transformation of intact bacterial cells by using the enteric pathoge...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Miller, J F, Dower, W J, Tompkins, L S
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1988
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279654/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3277182/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.3.856
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