Enzyme stimulation upon fertilization is revealed in electrically permeabilized sea urchin eggs.
Sea urchin eggs and embryos subjected to high-voltage electric discharge in a medium mimicking the intracellular milieu retain their structural integrity and remain permeable, permitting substrates to enter the cytoplasm and thus assay of enzyme activity. At saturating concentrations of substrates,...
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1988
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279645/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3422463/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.3.812 |
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