Enzyme stimulation upon fertilization is revealed in electrically permeabilized sea urchin eggs.
Sea urchin eggs and embryos subjected to high-voltage electric discharge in a medium mimicking the intracellular milieu retain their structural integrity and remain permeable, permitting substrates to enter the cytoplasm and thus assay of enzyme activity. At saturating concentrations of substrates,...
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| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1988
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279645/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3422463/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.3.812 |
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