In vitro transcription of eukaryotic genes is affected differently by the degree of DNA supercoiling.
In a posterior silk gland extract, covalently closed circular (ccc) DNA is in a superhelical state that supports more transcription of fibroin gene than does linear DNA. A HeLa cell extract showed neither the supercoiling activity nor the preference for the transcription of ccc DNA over linear DNA....
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1988
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279626/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2829200/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.3.718 |
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