Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: isolation and characterization of viable and nonviable mutants.

By following a strategy of genetic analysis of poliovirus, we have constructed a synthetic "mutagenesis cartridge" spanning the genome-linked viral protein coding region and flanking cleavage sites in an infectious cDNA clone of the type 1 (Mahoney) genome. The insertion of new restriction sites wit...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Kuhn, R J, Tada, H, Ypma-Wong, M F, Dunn, J J, Semler, B L, Wimmer, E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279582/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2829191/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.2.519
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