Construction of a "mutagenesis cartridge" for poliovirus genome-linked viral protein: isolation and characterization of viable and nonviable mutants.

By following a strategy of genetic analysis of poliovirus, we have constructed a synthetic "mutagenesis cartridge" spanning the genome-linked viral protein coding region and flanking cleavage sites in an infectious cDNA clone of the type 1 (Mahoney) genome. The insertion of new restriction...

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Autores principales: Kuhn, R J, Tada, H, Ypma-Wong, M F, Dunn, J J, Semler, B L, Wimmer, E
Formato: Artigo
Lenguaje:Inglês
Publicado: 1988
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC279582/
https://ncbi.nlm.nih.gov/pubmed/2829191
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