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Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231

Restriction–modification controller proteins play an essential role in regulating the temporal expression of restriction–modification genes. The controller protein C.Csp231I represents a new class of controller proteins. The gene was sublconed to allow overexpression in Escherichia coli. The protein...

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Detalhes bibliográficos
Main Authors: Streeter, S. D., McGeehan, J. E., Kneale, G. G.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2795595/
https://ncbi.nlm.nih.gov/pubmed/19724127
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109028681
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