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Crystallization and preliminary X-ray analysis of 4-­pyridoxolactonase from Mesorhizobium loti

4-Pyridoxolactonase from Mesorhizobium loti MAFF303099 has been overexpressed in Escherichia coli. The recombinant enzyme was purified and was crystallized by the sitting-drop vapour-diffusion method using PEG 4000 and ammonium sulfate as precipitants. Crystals of the free enzyme (form I) and of the...

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Bibliografiset tiedot
Päätekijät: Matsuda, Sayoko, Yokochi, Nana, Yoshikane, Yu, Kobayashi, Jun, Huy, Chu Nhat, Baba, Seiki, Kuramitsu, Seiki, Mikami, Bunzo, Yagi, Toshiharu
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: International Union of Crystallography 2009
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Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC2795592/
https://ncbi.nlm.nih.gov/pubmed/19724124
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109028772
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