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Crystallization and preliminary X-ray analysis of 4-pyridoxolactonase from Mesorhizobium loti
4-Pyridoxolactonase from Mesorhizobium loti MAFF303099 has been overexpressed in Escherichia coli. The recombinant enzyme was purified and was crystallized by the sitting-drop vapour-diffusion method using PEG 4000 and ammonium sulfate as precipitants. Crystals of the free enzyme (form I) and of the...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2795592/ https://ncbi.nlm.nih.gov/pubmed/19724124 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109028772 |
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