Introducing a true internal standard for the Comet assay to minimize intra- and inter-experiment variability in measures of DNA damage and repair

The Comet assay (CA) is a sensitive/simple measure of genotoxicity. However, many features of CA contribute variability. To minimize these, we have introduced internal standard materials consisting of ‘reference’ cells which have their DNA substituted with BrdU. Using a fluorescent anti-BrdU antibod...

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Bibliografiska uppgifter
Huvudupphovsmän: Zainol, Murizal, Stoute, Julia, Almeida, Gabriela M., Rapp, Alexander, Bowman, Karen J., Jones, George D. D.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Oxford University Press 2009
Ämnen:
Methods Online
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2794182/
https://ncbi.nlm.nih.gov/pubmed/19828597
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp826
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