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Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases

Two Arabidopsis xylosyltransferases, designated RGXT1 and RGXT2, were recently expressed in Baculovirus transfected insect cells and by use of the free sugar assay shown to catalyse transfer of D-xylose from UDP-α-D-xylose to L-fucose and derivatives hereof. We have now examined expression of RGXT1...

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Détails bibliographiques
Auteurs principaux: Petersen, Bent Larsen, Egelund, Jack, Damager, Iben, Faber, Kirsten, Krüger Jensen, Jacob, Yang, Zhang, Bennett, Eric Paul, Scheller, Henrik Vibe, Ulvskov, Peter
Format: Artigo
Langue:Inglês
Publié: Springer US 2009
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2793385/
https://ncbi.nlm.nih.gov/pubmed/19455420
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10719-009-9242-0
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