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Characterization of a Functional Soluble Form of a Brassica napus Membrane-Anchored Endo-1,4-β-Glucanase Heterologously Expressed in Pichia pastoris

The Brassica napus gene, Cel16, encodes a membrane-anchored endo-1,4-β-glucanase with a deduced molecular mass of 69 kD. As for other membrane-anchored endo-1,4-β-glucanases, Cel16 consists of a predicted intracellular, charged N terminus (methionine(1)-lysine(70)), a hydrophobic transmembrane domai...

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Autors principals: Mølhøj, Michael, Ulvskov, Peter, Dal Degan, Florence
Format: Artigo
Idioma:Inglês
Publicat: American Society of Plant Physiologists 2001
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC125102/
https://ncbi.nlm.nih.gov/pubmed/11598241
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