Reductive Glutaraldehydation of Amine Groups for Identification of Protein N-termini

The complete modification of protein amines by reductive glutaraldehydation is achieved in solution or in the gel in less than 15 minutes. Fragmentation of glutaraldehyde-modified proteins and peptides generates a1 fragment ions with enhanced intensity in MS/MS spectra. A method based on reductive g...

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Bibliographic Details
Main Authors: Russo, Allison, Chandramouli, Nagarajan, Zhang, Linqi, Deng, Haiteng
Format: Artigo
Language:Inglês
Published: 2008
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2793081/
https://ncbi.nlm.nih.gov/pubmed/18636758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr800224v
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