RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts

BACKGROUND: The quantitative polymerase chain reaction (qPCR) is a widely utilized method for gene-expression analysis. However, insufficient material often compromises large-scale gene-expression studies. The aim of this study is to evaluate an RNA pre-amplification method to produce micrograms of...

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Detalhes bibliográficos
Main Authors: Vermeulen, Joëlle, Derveaux, Stefaan, Lefever, Steve, De Smet, Els, De Preter, Katleen, Yigit, Nurten, De Paepe, Anne, Pattyn, Filip, Speleman, Frank, Vandesompele, Jo
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2009
Assuntos:
Short Report
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2789097/
https://ncbi.nlm.nih.gov/pubmed/19930725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-2-235
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