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Low-Cost Optical Lifetime Assisted Ratiometric Glutamine Sensor Based on Glutamine Binding Protein
Here we report a reagentless fluorescence sensing technique for glutamine in the submicromolar range based on the glutamine binding protein (QBP). The S179C mutant is labeled with the short-lived acrylodan (lifetime<5ns) and the long-lived tris(dibenzoylmethane) mono(5-amino-1,10-phenanthroline)e...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2008
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2783298/ https://ncbi.nlm.nih.gov/pubmed/18786501 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.08.018 |
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