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TU-tagging: cell type specific RNA isolation from intact complex tissues

We show that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil (4TU) delivery can be used to label and purify cell type specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell ty...

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Bibliografiske detaljer
Main Authors: Miller, Michael R., Robinson, Kristin J., Cleary, Michael D., Doe, Chris Q.
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2783170/
https://ncbi.nlm.nih.gov/pubmed/19430475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1329
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