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Role of Intramembrane Polar Residues in the YgfO Xanthine Permease: HIS-31 AND ASN-93 ARE CRUCIAL FOR AFFINITY AND SPECIFICITY, AND ASP-304 AND GLU-272 ARE IRREPLACEABLE
Using the YgfO xanthine permease of Escherichia coli as a bacterial model for the study of the evolutionarily ubiquitous nucleobase-ascorbate transporter (NAT/NCS2) family, we performed a systematic Cys-scanning and site-directed mutagenesis of 14 putatively charged (Asp, Glu, His, Lys, or Arg) and...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Biochemistry and Molecular Biology
2009
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2782019/ https://ncbi.nlm.nih.gov/pubmed/19581302 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.030734 |
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