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About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis
The influence of thiol blocking on the resolution of basic proteins by two-dimensional electrophoresis has been investigated. Cysteine blocking greatly increased resolution and decreased streaking, especially in the basic region of the gels. Two strategies of cysteine blocking were found efficient,...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Wiley-VCH
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2781085/ https://ncbi.nlm.nih.gov/pubmed/14997479 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200300589 |
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