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Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes

Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence recognition and adjust the DNA cleavage pattern. We have used time-resolved fluorescence spectroscopy of 2-...

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Hlavní autoři: Neely, Robert K., Tamulaitis, Gintautas, Chen, Kai, Kubala, Marta, Siksnys, Virginijus, Jones, Anita C.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777440/
https://ncbi.nlm.nih.gov/pubmed/19740769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp688
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