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In vivo expression and purification of aptamer-tagged small RNA regulators

Small non-coding RNAs (sRNAs) are an emerging class of post-transcriptional regulators of bacterial gene expression. To study sRNAs and their potential protein interaction partners, it is desirable to purify sRNAs from cells in their native form. Here, we used RNA-based affinity chromatography to pu...

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Main Authors: Said, Nelly, Rieder, Renate, Hurwitz, Robert, Deckert, Jochen, Urlaub, Henning, Vogel, Jörg
格式: Artigo
語言:Inglês
出版: Oxford University Press 2009
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777422/
https://ncbi.nlm.nih.gov/pubmed/19726584
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkp719
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