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Expression, purification and crystallization of an archaeal-type phosphoenolpyruvate carboxylase
An archaeal-type phosphoenolpyruvate carboxylase (PepcA) from Clostridium perfringens has been expressed in Escherichia coli in a soluble form with an amino-terminal His tag. The recombinant protein is enzymatically active and two crystal forms have been obtained. Complete diffraction data extending...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2777057/ https://ncbi.nlm.nih.gov/pubmed/19923749 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109042663 |
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