Llwytho...

Expression, purification and crystallization of an archaeal-type phosphoenolpyruvate carboxylase

An archaeal-type phosphoenolpyruvate carboxylase (PepcA) from Clostridium perfringens has been expressed in Escherichia coli in a soluble form with an amino-terminal His tag. The recombinant protein is enzymatically active and two crystal forms have been obtained. Complete diffraction data extending...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Dharmarajan, Lakshmi, Kraszewski, Jessica L., Mukhopadhyay, Biswarup, Dunten, Pete W.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: International Union of Crystallography 2009
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777057/
https://ncbi.nlm.nih.gov/pubmed/19923749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109042663
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