Purification, crystallization and preliminary X-ray analysis of β-glucosidase from Kluyveromyces marxianus NBRC1777

The intracellular β-glucosidase from Kluyveromyces marxianus NBRC1777 (KmBglI) belongs to glycoside hydrolase family 3 and has a unique domain architecture. Selenomethionine-labelled KmBglI was purified and crystallized by the hanging-drop vapour-diffusion method using the purified enzyme at 30 mg m...

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Detalhes bibliográficos
Main Authors: Yoshida, Erina, Hidaka, Masafumi, Fushinobu, Shinya, Koyanagi, Takashi, Minami, Hiromichi, Tamaki, Hisanori, Kitaoka, Motomitsu, Katayama, Takane, Kumagai, Hidehiko
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Crystallization Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2777056/
https://ncbi.nlm.nih.gov/pubmed/19923748
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109042948
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