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Single molecule detection of direct, homologous, DNA/DNA pairing

Using a parallel single molecule magnetic tweezers assay we demonstrate homologous pairing of two double-stranded (ds) DNA molecules in the absence of proteins, divalent metal ions, crowding agents, or free DNA ends. Pairing is accurate and rapid under physiological conditions of temperature and mon...

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Main Authors: Danilowicz, C., Lee, C. H., Kim, K., Hatch, K., Coljee, V. W., Kleckner, N., Prentiss, M.
格式: Artigo
語言:Inglês
出版: National Academy of Sciences 2009
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2775704/
https://ncbi.nlm.nih.gov/pubmed/19903884
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0911214106
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