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Facilitating Chromophore Formation of Engineered Ca(2+) Binding Green Fluorescent Proteins
Green fluorescent protein (GFP) containing a self-coded chromophore has been applied in protein trafficking and folding, gene expression, and as sensors in living cells. While the “cycle3” mutation denoted as C3 mutation (F99S/M153T/V163A) offer the ability to increase GFP fluorescence at 37 °C, it...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2774846/ https://ncbi.nlm.nih.gov/pubmed/19358822 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2009.03.016 |
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