Facilitating Chromophore Formation of Engineered Ca(2+) Binding Green Fluorescent Proteins

Green fluorescent protein (GFP) containing a self-coded chromophore has been applied in protein trafficking and folding, gene expression, and as sensors in living cells. While the “cycle3” mutation denoted as C3 mutation (F99S/M153T/V163A) offer the ability to increase GFP fluorescence at 37 °C, it...

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Main Authors: Holder, Angela N., Ellis, April L., Zou, Jin, Chen, Ning, Yang, Jenny J.
Format: Artigo
Jezik:Inglês
Izdano: 2009
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2774846/
https://ncbi.nlm.nih.gov/pubmed/19358822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2009.03.016
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