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Noninvasive estimation of cell cycle phase and proliferation rate of human mesenchymal stem cells by phase-shifting laser microscopy

The simultaneous determination of the cell cycle phase of individual adherent mesenchymal stem cells (MSCs) using a fluorescence microscope after staining with 4′,6-diamidine-2′-phenylindole dihydrochloride and bromodeoxyuridine and the laser phase shift by phase-shifting laser microscopy (PLM) reve...

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Main Authors: Tokumitsu, Ayako, Wakitani, Shigeyuki, Takagi, Mutsumi
Formato: Artigo
Idioma:Inglês
Publicado: Springer Netherlands 2009
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2774568/
https://ncbi.nlm.nih.gov/pubmed/19693683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-009-9209-9
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