Differential 14-3-3 Affinity Capture Reveals New Downstream Targets of Phosphatidylinositol 3-Kinase Signaling

We devised a strategy of 14-3-3 affinity capture and release, isotope differential (d(0)/d(4)) dimethyl labeling of tryptic digests, and phosphopeptide characterization to identify novel targets of insulin/IGF1/phosphatidylinositol 3-kinase signaling. Notably four known insulin-regulated proteins (P...

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Main Authors: Dubois, Fanny, Vandermoere, Franck, Gernez, Aurélie, Murphy, Jane, Toth, Rachel, Chen, Shuai, Geraghty, Kathryn M., Morrice, Nick A., MacKintosh, Carol
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2009
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Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2773716/
https://ncbi.nlm.nih.gov/pubmed/19648646
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M800544-MCP200
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