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Improved conditional expression systems resulting in physiological level of HNF4α expression confirm HNF4α induced apoptosis in the pancreatic β-cell line INS-1

BACKGROUND: To analyze gene function in mammalian cells tetracycline inducible expression of a gene-of-interest at a specific genomic location (Flp-In T-REx™) is most attractive. However, leakiness of basal transgene expression and artificially high expression level upon tetracycline addition may be...

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Hlavní autoři: Senkel, Sabine, Waldner, Christoph, Ryffel, Gerhart U, Thomas, Heike
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2009
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2768738/
https://ncbi.nlm.nih.gov/pubmed/19835622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-2-210
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