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The functional expression of toxic genes: Lessons learned from molecular cloning of CCH1, a high-affinity Ca(2+) channel
Some genes cannot be cloned by conventional methods because in most cases the genes or gene products are toxic to E. coli. CCH1 is a high-affinity Ca(2+) channel present in the plasma membrane of Cryptococcus neoformans and other fungi. Like many toxic genes, the molecular cloning of CCH1 has been a...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2009
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2767258/ https://ncbi.nlm.nih.gov/pubmed/19580778 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.06.039 |
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