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Quantification of Plasmid DNA Copies in the Nucleus after Lipoplex and Polyplex Transfection
Nuclear uptake of plasmid DNA is one of the many cellular barriers that limit the efficiency of non-viral gene delivery systems. We have determined the number of plasmids that reach the nucleus of a transfected cell using an internally standardized quantitative PCR (qPCR) assay. We isolated nuclei u...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2009
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2765102/ https://ncbi.nlm.nih.gov/pubmed/19211029 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jconrel.2008.12.016 |
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